Zusammenfassung

Skeletal muscle is the tissue most responsible for basal and exercise induced energy expenditure and is commonly investigated to provide insights into human physical performance and disease, such as obesity and diabetes.
Extend preliminary findings that mouse uncoupling protein-3 (UCP3) is increased in intermyofibrillar (IMF), but not in subsarcolommal (SS) mitochondria after bouts of swimming exercise. From this experiment we will measure mitochondrial uncouöling and mitochondrial ATP production rate (MAPR) in the SS an IMF mitochondrial populations after 1h of swimming exercise. This study will allow us to test the hypothesis that an increased UCP3 content will increase mitochondrial uncoupling and reduce the MAPR.