Determination of sildenafil, vardenafil and tadalafil metabolites in human urine by GC/MS
| Deutscher übersetzter Titel: | Die Bestimmung von Sildenafil-, Vardenafil- und Tadalafilmetaboliten in menschlichem Urin mittels GC/MS |
|---|---|
| Autor: | Strano Rossi, S.; Torre, X. de la; Molaioni, F.; Botrè, F. |
| Erschienen in: | Recent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop ; 28th Cologne Workshop on Dope Analysis , 7th to 12th March 2010 |
| Veröffentlicht: | Köln: Sportverl. Strauß (Verlag), 2010, S. 142, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Sammelwerksbeitrag |
| Medienart: | Gedruckte Ressource |
| Sprache: | Englisch |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201203002156 |
| Quelle: | BISp |
Abstract
The use of sildenafil analogues by athletes in order to increase the oxygen transport to tissues has been postulated. A method is described for the determination in urine of phosphodiesterase type 5 enzyme inhibitors (PDE5Is) sildenafil (SDF), vardenafil (VDF), and tadalafil (TDF) and their metabolites by gas chromatography - mass spectrometmy (GC-MS); the method involves liquid/liquid extraction of the analytes from urine and derivatisation to obtain trimethylsilyl derivatives. The metabolic profile was studied on real samples collected from subjects taking PDE5Is (Viagra®, Levitra® or Cialis®). The main urinary metabolites were identified and their MS fragmentation characterized. The optimal sample pre-treatment and GC/MS conditions for the detection of the metabolites have been studied, considering also their possible introduction in routine doping analysis. Verf.-Referat