Comparison of normal-phase and reverse-phase HPLC clean up for 13C/12C analysis of endogenous urinary steroids
Gespeichert in:
| Deutscher übersetzter Titel: | Vergleich der Probenreinigung bei Normalphase und Reverse Phase HPLC für die 13C/12C-Analyse von endogenen Steroiden im Urin |
|---|---|
| Autor: | Gougoulidis, V.; Piper, T.; Flenker, Ulrich; Schänzer, Wilhelm |
| Erschienen in: | Recent advances in doping analysis (17) : Proceedings of the Manfred Donike Workshop ; 27th Cologne Workshop on Dope Analysis ; 1st to 6th March 2009 |
| Veröffentlicht: | Köln: Sportverl. Strauß (Verlag), 2009, S. 189-192, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Sammelwerksbeitrag |
| Medienart: | Gedruckte Ressource |
| Sprache: | Englisch |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201101000571 |
| Quelle: | BISp |
Abstract
Due to the current shortage of acetonitrile the development of acetonitrile-saving methods in doping analysis is advisable. During sample preparation for 13C/12C analysis of endogenous urinary steroids by GC-C-IRMS mainly the HPLC clean up consumes acetonitrile. This is performed on a reversed phase column (RP). Acetonitrile constitutes the major part of the mobile phase. Therefore the HPLC method was migrated to a normal-phase (NP) column. n-Hexane and isopropanol make up the mobile phase. The method was originally developed for the clean up of norandrosterone samples. The aims of this study were to demonstrate sufficient agreement of both HPLC methods and to validate the NP method. None of the tested steroids failed. Verf.-Referat