Abstract

The list of prohibited substances by World Anti-Doping Agency (WADA) includes hundreds of compounds with variable chemical properties. Doping control laboratories have to be able to screen and identify these substances from a limited volume of biological sample at a limited time-frame. Therefore, the selection of an appropriate sample preparation method is important and mainly specifies the quantity and amount of target compounds. Extensive sample preparation procedures have been applied in fields of toxicology, environmental and food, as well as horse and human doping control analysis. The aim of this study was to develop a single sample preparation method for doping agents in a 1-mL aliquot of urine sample regardless of their chemical properties. The compounds were extracted with two mixed-mode solid phase extraction (SPE) columns with C8 and either strong cation or anion exchange mechanisms. The presented sample pretreatment method combining two separate SPE steps of HCX and HAX enables extensive doping screening covering most of the compound classes listed in WADA's code. All the materials required for the procedure were general laboratory regents and the total sample consumption was minimized to 1 mL. Simultaneous extraction of compounds with different chemical properties allowed the preparation of one single sample for LC-TOFMS analysis. Aus dem Text