A simplified and accurate method for the analysis of urinary metabolites of testosterone-related steroids using gas chromatography-combustion-isotope ratio mass spectrometry
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| Deutscher übersetzter Titel: | Eine vereinfachte und akurate Methode für die Analyse der Metaboliten von Testosteron-verwandten Steroiden mittels Gaschromatographie-Isotopenverhältnis-Massenspektrometrie |
|---|---|
| Autor: | Ouellet, Alexandre; LeBerre, Nicolas; Ayotte, Christiane |
| Erschienen in: | Recent advances in doping analysis (20) : Proceedings of the Manfred-Donike-Workshop ; 30th Cologne Workshop on Dope Analysis ; 26th February to 2nd March 2012 2. Variante |
| Veröffentlicht: | Köln: Sportverl. Strauß (Verlag), 2012, S. 76-81, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Sammelwerksbeitrag |
| Medienart: | Gedruckte Ressource |
| Sprache: | Englisch |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201308005788 |
| Quelle: | BISp |
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PU201308005788 |
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db |
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Literatur |
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2013-08-09T09:43:08Z |
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2013-08-06T11:22:20Z |
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BISp |
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Sammelwerksbeitrag |
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PU201308005770 |
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PU201308005770 |
| hierarchy_top_title |
Recent advances in doping analysis (20) : Proceedings of the Manfred-Donike-Workshop ; 30th Cologne Workshop on Dope Analysis ; 26th February to 2nd March 2012 2. Variante |
| hierarchy_parent_title |
Recent advances in doping analysis (20) : Proceedings of the Manfred-Donike-Workshop ; 30th Cologne Workshop on Dope Analysis ; 26th February to 2nd March 2012 2. Variante |
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PU201308005788 |
| is_hierarchy_title |
A simplified and accurate method for the analysis of urinary metabolites of testosterone-related steroids using gas chromatography-combustion-isotope ratio mass spectrometry |
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1 |
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bispincollection |
| publishDate |
2012 |
| publishDate_facet |
2012 |
| language |
eng |
| title |
A simplified and accurate method for the analysis of urinary metabolites of testosterone-related steroids using gas chromatography-combustion-isotope ratio mass spectrometry |
| spellingShingle |
A simplified and accurate method for the analysis of urinary metabolites of testosterone-related steroids using gas chromatography-combustion-isotope ratio mass spectrometry Biochemie Doping Dopinganalyse Dopingnachweis Gaschromatographie Massenspektrometrie Screening Steroid Testosteron Untersuchungsmethode Urin Urinausscheidung Urinuntersuchung |
| title_sort |
a simplified and accurate method for the analysis of urinary metabolites of testosteronerelated steroids using gas chromatographycombustionisotope ratio mass spectrometry |
| title_short |
A simplified and accurate method for the analysis of urinary metabolites of testosterone-related steroids using gas chromatography-combustion-isotope ratio mass spectrometry |
| title_alt |
Eine vereinfachte und akurate Methode für die Analyse der Metaboliten von Testosteron-verwandten Steroiden mittels Gaschromatographie-Isotopenverhältnis-Massenspektrometrie |
| title_alt_lang |
deu |
| media_type |
Gedruckte Ressource |
| city |
Köln |
| abstract |
The determination of the exogenous origin of urinary metabolites through the measurement by GC-C-IRMS of their carbon isotopic signature (δ13C) is widely used to confirm the administration of steroids related to testosterone and prohibited in sports. The rigorous analytical conditions required to perform accurate measurements have often dictated the selection of the final most intense metabolites, androsterone (A) and etiocholanolone (Etio), potentially limiting its efficacy. In fact, their alteration is less marked and persistent than for testosterone (T) itself and 5β-androstane-3α,17β-diol (5α-Adiol) or 5α-androstane-3α,17β-diol (5α-Adiol), which on the other end are present in much lower amount and hence, more difficult to test reliably. We have developed a one-step HPLC purification of seven diagnostic urinary metabolites (target steroids: TS, hydrolyzed glucuronides): T, DHEA, 5β-Adiol, 5α-Adiol, epitestosterone (E), A, Etio and two endogenous reference compounds (ERC), pregnanediol (pgdiol) and 5α-androst-16-en-3β-ol (16-enol). These steroids are pooled in three fractions and analyzed without derivatization. With regards to the GC-C-IRMS analysis, a multi-level isotopic calibration using the ‘identical treatment’ principle was found to provide results for purified reference steroids with a precision ≤ 0.17 and accuracy of ≤ 0.30 ‰(between assay, n=26). Compared to other common endogenous reference compounds, those selected in this study have δ13C values close to the testosterone metabolites which, along with the proposed isotopic calibration, produced reference intervals within ± 3 ‰ for most diagnostic TS-ERC pairs, in compliance with the requirements of the World Anti-Doping Agency. Verf.-Referat |
| abstract_lang |
eng |
| abstract_type |
abstract |
| author2 |
Ouellet, Alexandre LeBerre, Nicolas Ayotte, Christiane |
| author_facet |
Ouellet, Alexandre LeBerre, Nicolas Ayotte, Christiane |
| author2-role |
Autor Autor Autor |
| author2-authorityid |
|
| author_author_facet |
Ouellet, Alexandre LeBerre, Nicolas Ayotte, Christiane |
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|
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0/Autor/ 1/Autor/Ouellet, Alexandre/ 0/Autor/ 1/Autor/LeBerre, Nicolas/ 0/Autor/ 1/Autor/Ayotte, Christiane/ |
| publisher |
Sportverl. Strauß |
| publisher-role |
Verlag |
| publisher-address |
Köln |
| publisher-synonym |
|
| publisher-authorityid |
|
| publisher_publisher_facet |
Sportverl. Strauß |
| publisher_hierarchy_facet |
0/Verlag/ 1/Verlag/Sportverl. Strauß/ |
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0/Deutschland/ 1/Deutschland/Nordrhein-Westfalen/ |
| topic |
Biochemie Doping Dopinganalyse Dopingnachweis Gaschromatographie Massenspektrometrie Screening Steroid Testosteron Untersuchungsmethode Urin Urinausscheidung Urinuntersuchung |
| topic_facet |
Biochemie Doping Dopinganalyse Dopingnachweis Gaschromatographie Massenspektrometrie Screening Steroid Testosteron Untersuchungsmethode Urin Urinausscheidung Urinuntersuchung |
| spelling |
Biochemie Doping Dopinganalyse Dopingnachweis Gaschromatographie Massenspektrometrie Screening Steroid Testosteron Untersuchungsmethode Urin Urinausscheidung Urinuntersuchung biochemistry doping doping analysis doping detection gas chromatography investigation method mass spectrometry screening steroid testosterone urination urine urine examination Dopingmittel Dopinganalyse Dopinganalytik Leistungstest Untersuchungsmethodik Untersuchungsverfahren Methode Urinhormonausscheidung Urinhormonkonzentration Urinhormonmetabolitenkonzentration examination method urine, passage of urine, discharge of A simplified and accurate method for the analysis of urinary metabolites of testosterone-related steroids using gas chromatography-combustion-isotope ratio mass spectrometry PU201308005788 201308005788 |
| topic_en |
biochemistry doping doping analysis doping detection gas chromatography investigation method mass spectrometry screening steroid testosterone urination urine urine examination |
| topic_en_facet |
biochemistry doping doping analysis doping detection gas chromatography investigation method mass spectrometry screening steroid testosterone urination urine urine examination |
| synonym |
Dopingmittel Dopinganalyse Dopinganalytik Leistungstest Untersuchungsmethodik Untersuchungsverfahren Methode Urinhormonausscheidung Urinhormonkonzentration Urinhormonmetabolitenkonzentration |
| synonym_en |
examination method urine, passage of urine, discharge of |
| container_title |
Recent advances in doping analysis (20) : Proceedings of the Manfred-Donike-Workshop ; 30th Cologne Workshop on Dope Analysis ; 26th February to 2nd March 2012 2. Variante |
| container_start_page |
S. 76-81 |
| container_isbn |
3868840380 |
| has_references |
1 |
| institution |
BISp |
| _version_ |
1810937182689427456 |
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13,263502 |