Abstract

Several supplements have been purchased from two different internet-based suppliers and were analysed for their steroid content. Besides the findings of DHEA (and DHEA esters) products were found to contain 6-bromoandrostenedione, androstanoisoxazoles, and estra-4,9-diene-3,17-dione. Additionally ∆6-methyltestosterone was detected in the product "Jungle Warfare" from ALR Industries, androst-4-ene-3,11,17-trione in the product “11Oxo" from Ergopharm, and finally the product "Oxyguno" distributed by Spectra Force Research was found to contain 4-chloro-11-oxomethyltestosterone. These three compounds have been reported in the 1960s to show weak androgenic (about 1/2 of testosterone) and from weak to strong anabolic properties. The urinary metabolism was studied following the administration of one capsule to one test person each. The administration of ∆6-methyltestosterone was detectable for more than one week by monitoring the metabolites 17α-hydroxy-17ß-rnethylandrosta-4,6-dien-3-one, 17α-rnethyl-5ß-androstane-3α,17ß-diol, and 17ß-methyl-5ß-androstane-3α,17α-diol. Oral administration of androst-4-ene-3,11,17-trione resulted in several reduced metabolites with 11ß-hydroxyandrosterone as main product. Isotope ratio mass spectrometry confirmed the exogenous origin of 11ß-hydroxyandrosterone and 11ß-hydroxyetiocholanolone as well as their 11-oxo precursors for up to 24 hours. 4-Chloro-11ß-hydroxymethyltestosterone (4-chloro-11ß,17ß-dihydroxy-17α-methylandrost-4-en-3-one) was identified as main urinary metabolite following the ingestion of 4-chloro-11-oxomethyltestosterone. Verf.-Referat