Abstract

Stimulants, narcotics and anabolic steroids are named in the IAAF rules as dope agents. As the chemical structure of the dope agents varies to a large extend, all these substances cannot be detected only by one analytical method. But nevertheless, comprehensive screening procedures can be designed for closely related substances. These screening procedures are determined by the chemical and by the biochemical properties of these substances and can be outlined as followers: Screening procedure: 1. For volatile nitrogen containing compounds, excreted in the free form into the urine. 2. For nitrogen containing compounds, excreted conjugated as sulfates or Beta-glucurronides. 3. Fornitrogen containing compounds with extremely polar functional groups, detectable by high pressure liquid chromatography and UV-detection. 4. For anabolic steriods, excreted free or conjugated into the urine. Examples for the detection of stimulants, narcotic and anabolic steroids with the above listed screening procedures will be presented to illustrate the capability of these methods. Special precautions will be taken if an urine sample which shows up positive in a screening procedure. The urine sample will be analysed once more taking into account all the information received at the first screening procedure. Subsequently, enough analytical data will be collected to identify the substances unequivocally. Identification implies identy of all measured gas-chromatographic and mass spectrometric data. Verf.-Referat (gekuerzt)