LH detection by AutoDelfia and Vidas 3 immunoassays : total and intact LH comparison in a population of males tested for androgenic anabolic steroid abuse
| Deutscher übersetzter Titel: | LH-Nachweis durch AutoDelfia und Vidas 3 Immunoassays : totaler und intakter LH-Vergleich bei einer Population von Männern, die auf androgenen anabolen Steroidmissbrauch getestet wurden |
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| Autor: | Masquelier, M.; Tollbäck, P.; Palonek, E.; Ericsson, M. |
| Erschienen in: | Recent advances in doping analysis (24) : Proceedings of the Manfred Donike Workshop ; 34th Cologne Workshop on Dope Analysis ; 21st to 26th February 2016 |
| Veröffentlicht: | Köln: Sportverl. Strauß (Verlag), 2016, S. 79-82, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Sammelwerksbeitrag |
| Medienart: | Elektronische Ressource (Datenträger) |
| Dokumententyp: | Tagungsband |
| Sprache: | Englisch |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201709008164 |
| Quelle: | BISp |
Abstract des Autors
The release of luteinizing hormone (LH) is generally suppressed after intake of AAS such as testosterone due to negative feedback on the hypothalamic-pituitary-gonadal axis and could be used as indication of AAS misuse in cases where IRMS confirmation is not possible. WADA guidelines require assay of total LH, i.e. a/b heterodimers (intact LH), free b chain and b-core fragment. This study compared the use of total and intact urinary LH for identifying suspicious testosterone/epitestosterone (T/E) ratios, in 151 non-athlete samples, analyzed for workplace testing and abuse monitoring. Our data show that the measurement of intact LH is a possible alternative for total LH and that comparable results are achieved by the two techniques by using 250 for cut-off limit for the ratio T/intactLH (With T in nmol/L and LH in IU/L) or 0.5 (IU/L) as cut-off for LH after adjustment for specific gravity to 1.020 (sgLH). The choice of the optimal T/LH or sgLH cut-off shall be confirmed by complementary IRMS studies.