Synthesis, characterisation, and mass spectrometric detection of a pegylated EPO-mimetic peptide for sports drug testing purposes
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| Deutscher übersetzter Titel: | Synthese, Charakterisierung und massenspektrometrischer Nachweis eines pegylierten EPO-mimetischen Peptids zu Doping-Testzwecken |
|---|---|
| Autor: | Möller, Ines; Thomas, Andreas; Geyer, Hans; Schänzer, Wilhelm; Thevis, Mario |
| Erschienen in: | Rapid communications in mass spectrometry |
| Veröffentlicht: | 25 (2011), 15, S. 2115-2123, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Zeitschriftenartikel |
| Medienart: | Elektronische Ressource (online) Gedruckte Ressource |
| Sprache: | Englisch |
| ISSN: | 0951-4198, 1097-0231 |
| DOI: | 10.1002/rcm.5109 |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201410009726 |
| Quelle: | BISp |
| id |
PU201410009726 |
|---|---|
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db |
| format |
Literatur |
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2018-07-18T14:59:09Z |
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2014-10-21T13:42:05Z |
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BISp |
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Zeitschriftenartikel |
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JO00000100466 |
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JOI201100150100466 |
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Rapid communications in mass spectrometry |
| hierarchy_parent_title |
Rapid communications in mass spectrometry, 2011, 15 |
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PU201410009726 |
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Synthesis, characterisation, and mass spectrometric detection of a pegylated EPO-mimetic peptide for sports drug testing purposes |
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2115 |
| recordtype |
bisparticle |
| publishDate |
2011 |
| publishDate_facet |
2011 |
| language |
eng |
| title |
Synthesis, characterisation, and mass spectrometric detection of a pegylated EPO-mimetic peptide for sports drug testing purposes |
| spellingShingle |
Synthesis, characterisation, and mass spectrometric detection of a pegylated EPO-mimetic peptide for sports drug testing purposes Biochemie Blut Blutplasma Doping Dopinganalyse Dopingbekämpfung Dopingkontrolle Dopingnachweis Entwicklung Erythropoetin Flüssigkeitschromatographie Leistungssteigerung Massenspektrometrie Peptid Untersuchung, empirische |
| title_sort |
synthesis characterisation and mass spectrometric detection of a pegylated epomimetic peptide for sports drug testing purposes |
| title_short |
Synthesis, characterisation, and mass spectrometric detection of a pegylated EPO-mimetic peptide for sports drug testing purposes |
| title_alt |
Synthese, Charakterisierung und massenspektrometrischer Nachweis eines pegylierten EPO-mimetischen Peptids zu Doping-Testzwecken |
| title_alt_lang |
deu |
| media_type |
Elektronische Ressource (online) Gedruckte Ressource |
| city |
Chichester |
| abstract |
Erythropoietin (EPO) and other erythropoiesis-stimulating agents possess a high misuse potential in elite sport due to their ability to increase the oxygen transport capacity, which plays a vital role in enhancing endurance performance. Currently, a new generation of EPO-mimetic peptides is under development from which peginesatide (also referred to as Hematide™), a pegylated homodimeric peptide of approximately 45 kDa with no structural relationship to erythropoietin, is the most advanced drug candidate undergoing phase-III clinical trials. Since preventive doping research aims at the development of detection methods before a drug receives clinical approval, a selective and sensitive assay has to be established knowing that conventional doping control assays for EPO will not succeed in detecting peginesatide. Thus, a pegylated EPO-mimetic peptide simulating the structure and properties of the lead drug candidate peginesatide was synthesised as a model compound for this new class of emerging drugs and characterised by means of gel electrophoresis, matrix-assisted laser desorption/ionisation (MALDI) mass spectrometry, as well as liquid chromatography/electrospray ionisation tandem mass spectrometry (LC/ESI-MS/MS) after proteolytic digestion. Based on these results, a mass spectrometric detection method of the product in plasma was developed targeting a pentapeptide fragment after protein precipitation and subtilisin digestion. Its fitness for purpose was evaluated by the determination of selected method characteristics focusing particularly on specificity, recovery (ca. 60%), and limit of detection (1 ng/mL). Verf.-Referat |
| abstract_lang |
eng |
| abstract_type |
author |
| author2 |
Möller, Ines Thomas, Andreas Geyer, Hans Schänzer, Wilhelm Thevis, Mario |
| author_facet |
Möller, Ines Thomas, Andreas Geyer, Hans Schänzer, Wilhelm Thevis, Mario |
| author2-role |
Autor Autor Autor Autor Autor |
| author2-authorityid |
P136153 P26041 P72 P122169 |
| author_author_facet |
Möller, Ines Thomas, Andreas Geyer, Hans Schänzer, Wilhelm Thevis, Mario |
| author2-synonym |
Schaenzer, Wilhelm |
| author2_hierarchy_facet |
0/Autor/ 1/Autor/Möller, Ines/ 0/Autor/ 1/Autor/Thomas, Andreas/ 0/Autor/ 1/Autor/Geyer, Hans/ 0/Autor/ 1/Autor/Schänzer, Wilhelm/ 0/Autor/ 1/Autor/Thevis, Mario/ |
| doi |
10.1002/rcm.5109 |
| spelling |
10.1002/rcm.5109 0951-4198 1097-0231 RCMSEF 58731-x Biochemie Blut Blutplasma Doping Dopinganalyse Dopingbekämpfung Dopingkontrolle Dopingnachweis Entwicklung Erythropoetin Flüssigkeitschromatographie Leistungssteigerung Massenspektrometrie Peptid Untersuchung, empirische biochemistry blood blood plasma development doping doping analysis doping control doping detection empirical investigation erythropoietin fight against doping liquid chromatography mass spectrometry peptide performance increase Bluthormonkonzentration Plasma Dopingmittel Dopinganalytik Dopinganalyse EPO Leistungsoptimierung Leistungsverbesserung Leistungszuwachs Entfaltung Entwicklungsprozess Entwicklungssystem Empirie Empirismus Untersuchung, experimentelle Untersuchung increase in performance empirical study Synthesis, characterisation, and mass spectrometric detection of a pegylated EPO-mimetic peptide for sports drug testing purposes PU201410009726 201410009726 |
| url |
https://dx.doi.org/10.1002/rcm.5109 https://onlinelibrary.wiley.com/doi/abs/10.1002/rcm.5109 |
| url-type |
fulltext fulltext |
| url-free |
0 0 |
| issn |
0951-4198 1097-0231 |
| coden |
RCMSEF |
| zdb |
58731-x |
| location_hierarchy_facet |
0/Großbritannien/ 1/Großbritannien/England/ |
| topic |
Biochemie Blut Blutplasma Doping Dopinganalyse Dopingbekämpfung Dopingkontrolle Dopingnachweis Entwicklung Erythropoetin Flüssigkeitschromatographie Leistungssteigerung Massenspektrometrie Peptid Untersuchung, empirische |
| topic_facet |
Biochemie Blut Blutplasma Doping Dopinganalyse Dopingbekämpfung Dopingkontrolle Dopingnachweis Entwicklung Erythropoetin Flüssigkeitschromatographie Leistungssteigerung Massenspektrometrie Peptid Untersuchung, empirische |
| topic_en |
biochemistry blood blood plasma development doping doping analysis doping control doping detection empirical investigation erythropoietin fight against doping liquid chromatography mass spectrometry peptide performance increase |
| topic_en_facet |
biochemistry blood blood plasma development doping doping analysis doping control doping detection empirical investigation erythropoietin fight against doping liquid chromatography mass spectrometry peptide performance increase |
| synonym |
Bluthormonkonzentration Plasma Dopingmittel Dopinganalytik Dopinganalyse EPO Leistungsoptimierung Leistungsverbesserung Leistungszuwachs Entfaltung Entwicklungsprozess Entwicklungssystem Empirie Empirismus Untersuchung, experimentelle Untersuchung |
| synonym_en |
increase in performance empirical study |
| journal_facet |
Rapid communications in mass spectrometry |
| container_title |
Rapid communications in mass spectrometry |
| container_volume |
25 |
| container_issue |
15 |
| container_start_page |
S. 2115-2123 |
| has_references |
1 |
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BISp |
| journal_fac |
JO00000100466 |
| journal_year |
2011 |
| journal_issue |
15 |
| _version_ |
1791513715677331456 |
| score |
13,560136 |