TY  - JOUR
AU  - Beiter, Thomas
A2  - Beiter, Thomas
A2  - Fragasso, Annunziata
A2  - Hudemann, Jens
A2  - Nieß, Andreas Michael
A2  - Simon, Perikles
DB  - BISp
DP  - BISp
KW  - Bewegung
KW  - Biochemie
KW  - Blutplasma
KW  - Crosslauf
KW  - Desoxyribonukleinsäure
KW  - Laufband
KW  - Laufen
KW  - Mitochondrien
KW  - Sportmedizin
KW  - Untersuchung, empirische
KW  - Untersuchung, vergleichende
KW  - Zellkern
LA  - eng
TI  - Short-term treadmill running as a model for studying cell-free DNA kinetics in vivo
TT  - Kurzzeitiges Laufen auf dem Laufband als Modell für die Untersuchung von zellfreier DNA-Bewegung in vivo
PY  - 2011
N2  - BACKGROUND: Increased plasma concentrations of cell-free DNA (cf-DNA) are considered a hallmark of various clinical conditions. Despite intensive research in this field, limited data are available concerning the time course of release and clearance of cf-DNA in vivo. METHODS: We extracted cf-DNA from plasma samples taken before and immediately after a 10-km cross-country run, and from samples taken before, immediately after, and 30 min after exhaustive short-term treadmill exercise. The contribution of nuclear (nDNA) and mitochondrial DNA (mtDNA) was measured by quantitative real-time PCR. The incremental treadmill exercise setup was exploited to delineate the precise sequencing and timing of cf-nDNA, lactate, and high-mobility group box 1 protein (HMGB1) release during the exercise and recovery phases. RESULTS: Postexercise plasma cf-nDNA concentrations in cross-country and treadmill runners were significantly increased, by 7.6-fold and 9.9-fold, respectively (P < 0.001). cf-nDNA concentrations were not correlated with age, sex, or body mass index. Plasma concentrations of cf-nDNA and HMGB1 in postexercise samples of treadmill runners were significantly correlated (r = 0.84; P = 0.004). cf-mtDNA concentrations were not affected by treadmill exercise. Time-course analyses demonstrated that cf-nDNA is released within minutes after the onset of exercise and is rapidly cleared from the circulation after the cessation of exercise. Nearly congruent kinetics for cf-nDNA, lactate, and HMGB1 were observed during the exercise phase. CONCLUSIONS: A single bout of exhaustive short-term treadmill exercise constitutes a versatile model system suitable for addressing basic questions about cf-DNA biology.   Verf.-Referat
L2  - http://www.clinchem.org/content/57/4/633.full
L2  - https://dx.doi.org/10.1373/clinchem.2010.158030
DO  - 10.1373/clinchem.2010.158030
SP  - S. 633-636
SN  - 0009-9147
JO  - Clinical chemistry
IS  - 4
VL  - 57
M3  - Elektronische Ressource (online)
M3  - Gedruckte Ressource
ID  - PU201410009710
ER  -