Abstract

To investigate the effects on human growth hormone (GH) isoform ratios measured by World Anti-Doping Agency (WADA) approved kits, we analyzed the serum samples collected from volunteers of excretion study, athletes of routine anti-doping tests and patients anonymously provided by the hospitals. All serums were analyzed with the WADA approved kits following WADA guideline. We found that 1) the reactions of RecGH_1 from 53 pregnant women were competitively inhibited by some substances, 2) the situation of human placental lactogen (HPL) spiking test was consistent with the cross reactivity of the antibodies, 3) the analysis of the serum samples from acromegaly patients under the Glucose Tolerance Test indicated that the ratios may be effected by certain pathological conditions, 4) one of the 25 volunteers in our excretion study in 2010 showed that the Ratio_1 was much higher than Ratio_2 about 4 times, but Ratio_1 reanalyzed 2 years later was much lower than before maybe due to the long term storage or the different batches of Kit_1 or something else. Further investigation need to be implemented. Verf.-Referat