Abstract

Insulin and related synthetic analogues have been prohibited by the World Anti-Doping Agency (WADA) for athletes demonstrably not suffering from diabetes mellitus. Athletes could abuse insulin preparations to artificially improve their performance by increasing the muscle glycogen utilizing hyperinsulinaemic clamps prior to sports events or during recovery phases or by increasing muscle size inhibiting protein breakdown. A recent method describes an effective method, based on antibody-coated magnetic beads, nano-UPLC and Orbitrap mass spectrometry, for the purification and analysis of synthetic insulin analogs in urine samples. Here we preliminarily present a screening procedure based on the use of an HPLC-Chip interfaced with a quadrupole time-of-flight mass spectrometry system to detect the abuse of synthetic insulins. We tested the effectiveness of this approach on blank urine samples spiked with three synthetic insulins (Novolog® Apidra® and NovoRapid®). Einleitung