Abstract des Autors

Industrial, pharmaceutical and biochemical applications for pro-teases have a long history and proteolytic processes have beenexamined and understood for decades. [1] In contrast, the adulteration of urine samples in sports drug testing may represent a moreurgent and recent phenomenon in the detection of protein-basedprohibited substances. [2 – 5] Nowadays, enormous amounts of various peptidases are produced in bacteria in order to customizeevery proteolytic problem in industrial or biochemical implementation. Universal mixtures of proteases for optimal digestion underdifferent conditions (such as pH, temperature and time of activity) are commercially available allowing proteolysis under all kindsof external conditions. Cheating sportsmen thus have a large variety of effective proteases to choose from, which needs to betaken into account when conducting doping analysis of protein-based performance-enhancing agents. This case report presentsthe sodium dodecyl sulphate – polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-mass spectrometry (LC-MS) approaches for the detection of manipulation using a proteasein doping-control urine samples.