Temporal indication of cannabis use by means of THC glucuronide determination
Deutscher übersetzter Titel: | Zeitlicher Befund von Cannabis durch Bestimmung der THC-Glucuronide |
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Autor: | Mareck, Ute; Haenelt, Nadine; Geyer, Hans; Guddat, Sven; Kamber, Matthias; Brenneisen, Rudolf; Thevis, Mario; Schänzer, Wilhelm |
Erschienen in: | Drug testing and analysis |
Veröffentlicht: | 1 (2009), 11/12, S. 505 - 510, Lit. |
Format: | Literatur (SPOLIT) |
Publikationstyp: | Zeitschriftenartikel |
Medienart: | Gedruckte Ressource Elektronische Ressource (online) |
Sprache: | Englisch |
ISSN: | 1942-7603, 1942-7611 |
DOI: | 10.1002/dta.106 |
Schlagworte: | |
Online Zugang: | |
Erfassungsnummer: | PU201003002685 |
Quelle: | BISp |
Abstract
According to the regulations of the World Anti-Doping Agency (WADA), the use of cannabinoids is forbidden in competition. In doping controls, the detection of cannabinoid misuse is based on the analysis of the non-psychoactive metabolite 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (carboxy-THC). The determination of values greater than 15 ng/mL in urine represents an adverse analytical finding; however, no accurate prediction of the time of application is possible as the half-life of carboxy-THC ranges between three and four days. Consequently the detection of carboxy-THC in doping control urine samples collected in competition might also result from cannabis use in out-of-competition periods. The analysis of the glucuronide of the pharmacologically active delta 9-tetrahydrocannabinol (THC-gluc) may represent a complementary indicator for the detection of cannabis misuse in competition. An assay for the determination of THC-gluc in human urine was established. The sample preparation consisted of liquid-liquid extraction of urine specimens, and extracts were analysed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Authentic doping-control urine samples as well as specimens obtained from a controlled smoking study were analysed and assay characteristics such as specificity, detection limit (0.1 ng/mL), precision (>90%), recovery (80%), and extraction efficiency (90%) were determined. Verf.-Referat