Abstract

GC-MS plays an important role in doping control. Following proceedings of previous workshops the products of Agilent Technologies are widespread in doping control laboratories. Since the introduction of the fast GC-MS equipment evolution has resulted in the availability of benchtop instruments, monolithic quadrupoles, gold plated quadrupoles and inert sources. In 2006, a new instrument was marketed (MSD 5975) with several new features compared to previous series. Besides the introduction of a window on the mass spectrometer allowing accurate column positioning, the most important improvement was the introduction of high performance electronics allowing a faster data transfer and a higher scan rate. In addition, the high data transfer permits the alternative acquisition of SIM and scan data. At present analytical methods in doping control using gas chromatography are used for the detection of anabolic androgenic steroids, narcotic agents and stimulants. While in the past GC-NPD was often used to determine volatile nitrogen containing stimulants such as amphetamine, a comprehensive GC-MS screening method is now available for the simultaneous detection of narcotic agents and stimulants using MSTFA derivatisation. Additionally a second analytical method is used to detect anabolic steroids in urine (traditionally called extraction procedure IV in doping control laboratories). While the former is performed in the scan mode the latter uses selected ion monitoring (SIM). As the new generation of analytical equipment allows for the simultaneous acquisition of SIM and scan data a combination of the currently used analytical methods could result in an increased productivity and reduce the total analytical run time. Therefore the possibilities of SIM/scan in doping control was evaluated. Einleitung (geändert)