Abstract

The objective of this study is to demonstrate that the criteria fixed by the WADA (TD2004EAAS) related to the passage from GC/MS analysis to GC/C/IRMS has not been fulfilled after oral administration of androstenedione. This study suggests the investigation of additional criteria in GC/C/IRMS analysis based on the difference between δ13C of androsterone, etiocholanolone and 5δdiol, 5ßdiol respectively. The results indicate that GC/C/RMS analysis has proven to be an efficient tool for anti-doping control of testosterone and/or its precursors. It is based on the calculation of the difference between δ13C values of their metabolites and the ERC (16(5α)-androsten-3α-ol) and Pdiol. This analysis has yielded to the fact that the sample remains positive for 60 hours; however, the passage criteria from GC/MS analysis to the GC/C/IRMS one are no longer fulfilled in a 20 hours time following the oral administration of androstenedione. This study has focused on the calculation of the differences between δ13C-values of androsterone and etiocholanolone as well as between 5αdiol and 5ßdiol and proved that they have become more important in the case of androstenedione administration. We suggest and taking account of these experiments results to adopt those differences as an additional criterion in GC/C/IRMS analysis. This study can be extended and enriched by multiplying the number of volunteers and diversifying the population to establish a rule in order to harmonize the interpretation of isotopic measurement and to eliminate confusions and doubts. Aus dem Text (geändert)