Screening for N-desmethyl- and N-bisdesmethyl metabolites of Sibutramine using liquid chromatography-tandem mass spectrometry
Gespeichert in:
| Deutscher übersetzter Titel: | Das Screening von N-desmethyl- und N-bisdesmethyl-Metaboliten von Sibutramin mittels Flüssigkeits-Chromatographie Tandem Massenspektrometrie |
|---|---|
| Autor: | Sigmund, G.; Thevis, Mario; Schiffer, A.K.; Schänzer, Wilhelm |
| Erschienen in: | Recent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis ; 4th to 9th June 2006 |
| Veröffentlicht: | Köln: Sportverl. Strauß (Verlag), 2006, S. 403-406, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Sammelwerksbeitrag |
| Medienart: | Elektronische Ressource (online) Gedruckte Ressource |
| Sprache: | Englisch |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201001000265 |
| Quelle: | BISp |
Abstract
Since January 2006, the list of prohibited substances established by the World Anti-Doping Agency includes the antidepressant / anti-obesity drug Sibutramine. Due to its rapid degradation to its active metabolites N-desmethyl and N-bisdesmethyl Sibutramine, reference compounds were synthesized and included into an existing screening assay to allow the unambiguous determination of these metabolic products in human urine using liquid-liquid extraction followed by liquid chromatography – tandem mass spectrometry. Characteristic product ions, obtained after electrospray ionization and collision-induced dissociation, were elucidated using high resolution / high accuracy mass measurements with a hybrid linear ion trap / orbitrap mass analyzer. Einleitung