Comprehensive screening of urinary free fraction using HPLC-MS/MS
Gespeichert in:
| Deutscher übersetzter Titel: | Ein umfassendes Screening von freien Fraktionen im Urin mittels HPLC-MS/MS |
|---|---|
| Autor: | Dikunets, M.A.; Appolonova, S.A.; Rodchenkov, G.M. |
| Erschienen in: | Recent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis ; 24th to 29th February 2008 |
| Veröffentlicht: | Köln: Sportverl. Strauß (Verlag), 2008, S. 293-296, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Sammelwerksbeitrag |
| Medienart: | Elektronische Ressource (online) Gedruckte Ressource |
| Sprache: | Englisch |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU200909005090 |
| Quelle: | BISp |
Abstract
Two high-throughput high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) methods were developed for the screening of five important classes of drugs in sports, namely 23 corticosteroids, 12 anabolic agents, 23 diuretics, 49 stimulants and narcotics. Sample preparation included liquid-liquid extraction, evaporation of organic layer in nitrogen stream and reconstitution of dry residue. Thereafter two HPLC-MS/MS injections from the same vial (aliquot) were done with different mode of registration: negative ions for corticosteroids and most diuretics, and positive ions for some diuretics, anabolic steroids, narcotics and stimulants. Verf.-Referat