Analysis of human urinary proteins after physical exercise by two-dimensional electrophoresis under non-denaturing conditions
| Deutscher übersetzter Titel: | Analyse der belastungsbedingten Proteinausscheidung im menschlichen Harn mit Hilfe zweidimensionaler Elektrophorese unter nicht-denaturierenden Bedingungen |
|---|---|
| Autor: | Hiro, Takumitsu; Inoue, Tatsuki; Tomioka, Toru; Hirota, Koichi; Kadofuku, Tsuyoki; Makino, Yoshiaki; Sato, Tsuneo; Takeda, Minoru |
| Erschienen in: | Japanese journal of physical fitness and sports medicine |
| Veröffentlicht: | 42 (1993), 2, S. 138-144, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Zeitschriftenartikel |
| Medienart: | Gedruckte Ressource |
| Sprache: | Englisch |
| ISSN: | 0039-906X, 1881-4751 |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU199401068551 |
| Quelle: | BISp |
Abstract
A study was conducted to investigate the changes in human urinary proteins caused by physical exercise. Nine subjects (male middle-distance runners, 19 - 21 years) were loaded by physical exercise with a 10 km-run, and quantitative and qualitative changes in urinary proteins were examined using various techniques including polyacrylamide gel electrophoresis, immunoelectrophoresis and two-dimensional electrophoresis. Total protein concentrations in urine from all subjects before exercise were very low (0.68 - 2.56 mg/dl), but those were increase remarkably after exercise. Few proteins except for albumin were detected in urine before run bypolyacrylamide gel electrophoresis and immunoelectrophoresis, however, a few proteins were observed after exercise in addition to a marked increase of albumin. Several proteins which were not observed in serum could also be detected in urine after exercise by two-dimensional electrophoresis under non-denaturing condition. It is considered that two-dimensional electrophoresis is a very powerful technique for analysing dilute and complicated protein mixture in biological fluids such as urine. Verf.-Referat