Analysis of Bumetanide in human urine by high-performance liquid chromatography with fluorescence detection and gas chromatography/mass spectrometry
Gespeichert in:
| Deutscher übersetzter Titel: | Analyse von Bumetanid im menschlichen Urin mittels Hochleistungs-Fluessigkeitschromatographie mit Fluoreszenznachweis und mittels Gaschromatographie/Massenspektrometrie |
|---|---|
| Autor: | Gradeen, Claire Y.; Billay, Debbie M.; Chan, Siu C. |
| Erschienen in: | Journal of analytical toxicology |
| Veröffentlicht: | 14 (1990), 2, S. 123-126, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Zeitschriftenartikel |
| Medienart: | Gedruckte Ressource |
| Sprache: | Englisch |
| ISSN: | 0146-4760, 1945-2403 |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU199102037292 |
| Quelle: | BISp |
Abstract
Bumetanide is a potent diuretic. Its concentration in urine after therapeutic doses is in the low ng/mL range. A procedure using reversed-phase HPLC with fluorescence detection is described. Using this procedure, the authors have been able to measure bumetanide in a urine sample at 10 ng/mL with a signal-to-noise ratio of 7. Therefore, the detection limit is lower than this concentration. Confirmation is by a GC/MS procedure after methylating the diuretic. Derivatization is by extractive alkylation with tetrabutylammonium hydrogen sulfate as the phase transfer reagent and iodomethane as the methylating agent. Verf.-Referat