Swimming training potentiates the recovery of femoral neck strength in young diabetic rats under insulin therapy

Author: de Jesus Gomes, Gilton; Carlo, Ricardo Junqueira Del; da Silva, Márcia Ferreira; da Cunha, Daise Nunes Queiroz; da Silva, Edson; da Silva, Karina Ana; Carneiro-Junior, Miguel Araújo; Prímola-Gomes, Thales Nicolau; Natali, Antônio José
Language: English
Published: 2019
Source: PubMed Central (PMC)
Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474315/
https://doi.org/10.6061/clinics/2019/e829
Identification number: ftpubmed:oai:pubmedcentral.nih.gov:6474315

Summary

OBJECTIVE: To test whether swimming training benefits femoral neck strength in young diabetic rats under insulin therapy. METHODS: A total of 60 male Wistar rats (age: 40 days) were divided equally into the following six groups: control sedentary, control exercise, diabetic sedentary, diabetic exercise, diabetic sedentary plus insulin and diabetic exercise plus insulin. Diabetes was induced with a unique intraperitoneal injection (60 mg/kg body weight) of streptozotocin. Seven days after the injection and after 12 hours of fasting, the animals with blood glucose levels ≥300 mg/dL were considered diabetic. Seven days after the induction of diabetes, the animals in the exercise groups were subjected to progressive swimming training (final week: 90 min/day; 5 days/week; 5% load) for eight weeks. The animals in the insulin groups received a daily dose of insulin (2-4 U/day) for the same period. RESULTS: Severe streptozotocin-induced diabetes reduced the structural properties of the femoral neck (trabecular bone volume, trabecular thickness and collagen fiber content). The femoral neck mechanical properties (maximum load and tenacity) were also impaired in the diabetic rats. Insulin therapy partially reversed the damage induced by diabetes on the structural properties of the bone and mitigated the reductions in the mechanical properties of the bone. The combination of therapies further increased the femoral neck trabecular bone volume (∼30%), trabecular thickness (∼24%), collagen type I (∼19%) and type III (∼13%) fiber contents, maximum load (∼25%) and tenacity (∼14%). CONCLUSIONS: Eight weeks of swimming training potentiates the recovery of femoral neck strength in young rats with severe streptozotocin-induced diabetes under insulin therapy.