Zusammenfassung

ObjectiveTo investigate the changes in the function of dendritic cells (DCs) after HCV adherence, possible mechanisms of HCV adherence of DCs mediated by CLEC4M, and the impact of CLEC4M on innate immune response mediated by DCs. MethodsPeripheral blood mononuclear cells (PBMCs) were isolated from healthy people and induced to differentiate into mature DCs. Flow cytometry was used to measure the expression of CLEC4M on the surface of DCs. Mature DCs were infected by HCVcc and positive control group, mannan interference group, CLEC4M monoclonal antibody interference group, and blank control group were established. The supernatant was collected from each infected group, and ELISA was used to measure the changes in the levels of interleukin-10 (IL-10) and interleukin-12p70 (IL-12p70) in supernatant. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between any two groups. ResultsThe positive rate of CLEC4M in the surface of DCs reached 82.6%. There were significant differences in the levels of IL-10 and IL-12p70 between the groups (F=5446 and 77.72, both P＜0.001). The positive control group had significantly higher levels of IL-10 and IL-12p70 than the two interference groups (all P＜0.001), and compared with the blank control group, the positive control group, CLEC4M monoclonal antibody interference group, and mannan interference group had significantly higher levels of IL-10 and IL-12p70 (all P＜0.001). ConclusionCLEC4M mediates HCV adherence of DCs, participates in the body′s immune response, and regulate the expression of DC immune molecules.