Antibacterial activity of calcium hydroxide combined with triple antibiotic paste against enterococcus faecalis; an ın vitro study
Autor: | Adem Kuşgöz; Erhan Özcan; İpek Arslan; Melek İnci |
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Sprache: | Englisch |
Veröffentlicht: |
2013 |
Quelle: | Directory of Open Access Journals: DOAJ Articles |
Online Zugang: |
https://dergipark.org.tr/tr/download/article-file/48353 https://doaj.org/toc/1302-5805 https://doaj.org/toc/2146-2852 1302-5805 2146-2852 doi:10.7126/cdj.2012.1571 https://doaj.org/article/82f56f3f9add4cce8d57aa869ea81bc5 https://doi.org/10.7126/cdj.2012.1571 https://doaj.org/article/82f56f3f9add4cce8d57aa869ea81bc5 |
Erfassungsnummer: | ftdoajarticles:oai:doaj.org/article:82f56f3f9add4cce8d57aa869ea81bc5 |
Zusammenfassung
Objectives: The aim of the present in vitro study was to investigate the antibacterial efficacy of calcium hydroxide mixed with triple antibiotic paste at various rates. Materials and Methods: Triple antibiotic paste (TAP; metronidizol, minocycline, ciprofloxacin) and calcium hydroxide powder was mixed in different proportions and tested for antibacterial activity against E. faecalis (ATCC 29212)using the agar diffusion method. The samples were divided into 10 different groups;Group 1: 100% Ca(OH)2, Group 2: 99,5% Ca(OH)2 + 0.5% TAP, Group 3: 99% Ca(OH)2 + 1%TAP, Group 4: 97,5% Ca(OH)2 + 2,5% TAP, Group 5: 95% Ca(OH)2 + 5%TAP, Group 6: 90% Ca(OH)2 + 10%TAP, Group 7: 75% Ca(OH)2 + 25% TAP, Group 8: 50% Ca(OH)2 + 50% TAP, Group 9: 25% Ca(OH)2 + 75% TAP, Group 10: 0 TAP. Standard holes in the cultivated agar plates were filled with one of the samples and the zones of microbial inhibition were measured after incubation period (48 hours). The experiment was performed 8 times with each medicament. Results: Group 1 had no effect against E.faecalis. Group 10 demonstrated high antibacterial activity than any other groups in this study. The antibacterial effects of the groups could be ranked from strongest to weakest as follows: Group 10, 9, 8, 7, 6, 5, 4, 3, 2, 1. Conclusions: Adding the triple antibiotic paste increased he antibacterial efficacy of calcium hydroxide. In addition, the antibacterial effect of calcium hydroxide also increased with higher proportion of TAP.