Zusammenfassung

OBJECTIVES The study aimed to investigate the effects of the exercise training on inflammatory gene expression in adipose tissue via aging and obesity. METHODS The study subjects were forty 10-week old and 70-week old male Wistar rats. The young and old-aged rats were divided into four groups of ten: control (non-exercise) group (Y-Non; O-Non) and exercise group (Y-Ex; O-Ex). After the exercise training, intergroup differences in body composition, oral glucose tolerance test result, and protein expression levels of IL-6, TNFα, IL-10, and IL-4 in adipose tissue, were compared. RESULTS Both body weight and body fat-free mass were lower in old-aged rats than young rats; O-Non group, in particular, displayed the highest body fat mass and lowest fat-free mass. In oral glucose tolerance test result, old-aged rats exhibited higher blood glucose and insulin concentrations than young rats. TNF-α protein expression was higher in old-aged rats than young rats with the Y-Ex group displaying the lowest expression. IL-6 protein expression was significantly lower in the exercise group than in the control group for both old-aged and young rats (p < 0.05). IL-10 protein expression was not significantly different between the exercise group and the control group for either old-aged or young rats. IL-4 protein expression was significantly lower in the exercise group of young rats (p < 0.05). CONCLUSIONS Based on these findings, the analysis of the effects of exercise training on inflammatory gene expression in the adipose tissue of obese old-aged rats showed that the exercise training has effectively promoted the shift to M2 of cytokines secretion pattern related to obesity-induced pro-inflammation in old-aged rats and helped to improve insulin sensitivity.