Effects of arecoline and calcium ion on the permeability of dysplastic oral epithelia model in vitro
Autor: | HOU Dong⁃ lan; CHEN Rong; GAO Yi⁃jun; YIN Xiao⁃min |
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Sprache: | Chinesisch |
Veröffentlicht: |
2017 |
Quelle: | Directory of Open Access Journals: DOAJ Articles |
Online Zugang: |
http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2017.01.004 https://doaj.org/toc/2096-1456 doi:10.12016/j.issn.2096⁃1456.2017.01.004 2096-1456 https://doaj.org/article/03d53acf448640058cbf874b275054c4 https://doi.org/10.12016/j.issn.2096⁃1456.2017.01.004 https://doaj.org/article/03d53acf448640058cbf874b275054c4 |
Erfassungsnummer: | ftdoajarticles:oai:doaj.org/article:03d53acf448640058cbf874b275054c4 |
Zusammenfassung
Objective To study the effects of arecolineand Calcium ion (Ca2+) on the permeability of dysplastic oral epithelia model in vitro. Methods To establish the dysplastic oral epithelia model in vitro by culturing oral keratinocyte (DOK) and harvesting a DOK cell monolayer. The models were divided into control group, arecoline group and "Ca2++ are⁃ coline" group. The values of Lucifer Yellow Papp were used to estimate the permeability changes of models after treated⁃ with arecoline and Ca2+. Results In arecoline group, the lucifer yellow Papp values of each subgroup were higher than that of control group (P < 0.05), and the values increased as arecoline concentration elevated and time lasted (P < 0.05). When the "Ca2 ++ arecoline" group were pretreated with Ca2 +, the values of subgroup "10 μg/mL" was lower than that of the corresponding arecoline group (P > 0.05). However, the value of subgroup "4 h 10 μg/mL" of "Ca2++ arecoline" group had no statistical difference with that of control group (P > 0.05), while the other subgroups were increased (P < 0.05); Be⁃ sides, these values in "Ca2++ arecoline" group were increased as the arecoline concentration elevated and time lasted too (P < 0.05). Conclusion Intervention of arecoline contributes to the increase of permeability of DOK cell monolayer mod⁃ el, which maybe an important reason for the cancerization of dysplastic oral epithelia, however Ca2+might weaken these ef⁃ fects of arecoline in the process.