TY  - JOUR
AU  - Thomas, Andreas
A2  - Thomas, Andreas
A2  - Solymos, Emese
A2  - Schänzer, Wilhelm
A2  - Baume, Norbert
A2  - Saugy, Martial
A2  - Dellanna, Frank
A2  - Thevis, Mario
DB  - BISp
DP  - BISp
KW  - Biochemie
KW  - Doping
KW  - Dopingbekämpfung
KW  - Dopingkontrolle
KW  - Dopingnachweis
KW  - Flüssigkeitschromatographie
KW  - Gaschromatographie
KW  - Massenspektrometrie
KW  - Peptidhormon
KW  - Pharmakodynamik
KW  - Pharmakon
KW  - Protein
KW  - Sportmedizin
KW  - Steroid, anaboles
KW  - Stoffwechselprodukt
KW  - Untersuchung, empirische
KW  - Urinuntersuchung
LA  - eng
TI  - Determination of Vasopressin and Desmopressin in urine by means of liquid chromatography coupled to quadrupole time-of-flight mass spectrometry for doping control purposes
TT  - Bestimmung von Vasopressin und Desmopressin im Urin mittels der Flüssigchromatographie gekoppelt mit der Quadrupol-Massenspektrometrie zu Dopingkontrollzwecken
PY  - 2011
N2  - The anti-diuretic neurohypophysial hormone Vasopressin (Vp) and its synthetic analogue Desmopressin (Dp, 1-desamino-vasopressin) have received considerable attention from doping control authorities due to their impact on physiological blood parameters. Accordingly, the illicit use of Desmopressin in elite sport is sanctioned by the World Anti-Doping Agency (WADA) and the drug is classified as masking agent. Vp and Dp are small (8-9 amino acids) peptides administered orally as well as intranasally. Within the present study a method to determine Dp and Vp in urinary doping control samples by means of liquid chromatography coupled to quadrupole high resolution time-of-flight mass spectrometry was developed. After addition of Lys-Vasopressin as internal standard and efficient sample clean up with a mixed mode solid phase extraction (weak cation exchange), the samples were directly injected into the LC-MS system. The method was validated considering the parameters specificity, linearity, recovery (80-100%), accuracy, robustness, limit of detection/quantification (20/50 pg mL(-1)), precision (inter/intra-day<10%), ion suppression and stability. The analysis of administration study urine samples collected after a single intranasal or oral application of Dp yielded in detection windows for the unchanged target analyte for up to 20 h at concentrations between 50 and 600 pg mL(-1). Endogenous Vp was detected in concentrations of approximately 20-200 pg mL(-1) in spontaneous urine samples obtained from healthy volunteers. The general requirements of the developed method provide the characteristics for an easy transfer to other anti-doping laboratories and support closing another potential gap for cheating athletes.   Verf.-Referat
L2  - http://ac.els-cdn.com/S000326701101258X/1-s2.0-S000326701101258X-main.pdf?_tid=f6299ed4-5aa4-11e4-8748-00000aacb35d&acdnat=1414062708_a4fe91631b01bc25d01e169af1c9f001
L2  - http://www.sciencedirect.com/science/article/pii/S000326701101258X
L2  - https://dx.doi.org/10.1016/j.aca.2011.09.027
DO  - 10.1016/j.aca.2011.09.027
SP  - S. 107-113
SN  - 0003-2670
JO  - Analytica chimica acta
IS  - 1-2
VL  - 707
M3  - Elektronische Ressource (online)
M3  - Gedruckte Ressource
ID  - PU201410009826
ER  -