Detection of the misuse of testosterone gel in subjects with low basal Testosterone/Epitestosterone (T/E) value

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Bibliographic Details
Title translated into German:Nachweis des Missbrauchs von Testosterongel bei Personen mit niedrigen basalen Testosteron/Epitestosteron (T/E) Werten
Author:Jain, S.; Shrivastava, A.; Kaur, T.; Soni, A.; Haseen, J.; Nimker, V.; Beotra, A.
Published in:Recent advances in doping analysis (21) : Proceedings of the Manfred-Donike-Workshop ; 31th Cologne Workshop on Dope Analysis ; 24th February to 1st March 2013
Published:Köln: Sportverl. Strauß (Verlag), 2013, S. 19-25, Lit.
Format: Publications (Database SPOLIT)
Publication Type: Compilation article
Media type: Electronic resource (data carrier)
Language:English
Keywords:
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Identification number:PU201408007415
Source:BISp

Abstract

Testosterone is one of the most abused endogenous anabolic androgenic steroids in sports. Testosterone Gel (T-gel) applied transdermally, metabolizes in a different way keeping the concentration of other metabolites low as compared to 5-alpha metabolites. The aim of the present work was to study the effect of T-gel application on the endogenous steroid profile and δ13 values in subjects with low basal T/E ratio (<0.3). Two healthy male volunteers with low T/E ratios (<0.5) were selected. Urine samples were collected before, during and after T-gel application for 10 days. All urine samples were analysed for the endogenous steroid profile and δ13 values of endogenous steroids. The results of the T-gel excretion study of both volunteers revealed that the endogenous profile and their respective ratios were within the World Anti Doping Agency (WADA) prescribed limits. On the basis of the steroid profile, none of the sample qualified for GC-IRMS analysis as per existing and the proposed WADA technical document on endogenous steroids except one sample of volunteer-2. The most discriminating parameters of the steroid profile were the ratios Androsterone/Epitestosterone (Andro/E), 5α-androstane-3α,17β-diol/epitestosterone (5α-diol/E). For the GC-IRMS analysis, 5α-diol was the suitable target compound for long term detection of T-gel application. Individual reference ranges of the parameters of steroid profile were more suitable for the detection of misuse of T-gel application in subjects with low basal T/E values. Verf.-Referat