Detection of new clomiphene metabolites in human urine by liquid chromatography-quadrupole time-of-flight tandem mass spectrometry
| Deutscher übersetzter Titel: | Der Nachweis von neuen Clomifenmetaboliten in humanem Urin mittels Flüssigkeitschromatographie-Quadrupol-Flugzeit-Tandem-Massenspektrometrie |
|---|---|
| Autor: | Lu, Jianghai; He, Genye; Wang, Xiaobing; Xu, Youxuan; Wu, Yun; Dong, Ying; He, Zhenwen; Liu, Xin; Bo, Tao; Ouyang, Gangfeng |
| Erschienen in: | Recent advances in doping analysis (20) : Proceedings of the Manfred-Donike-Workshop ; 30th Cologne Workshop on Dope Analysis ; 26th February to 2nd March 2012 2. Variante |
| Veröffentlicht: | Köln: Sportverl. Strauß (Verlag), 2012, S. 170, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Sammelwerksbeitrag |
| Medienart: | Gedruckte Ressource |
| Sprache: | Englisch |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201308005818 |
| Quelle: | BISp |
Abstract
Clomiphene, a selective estrogen receptor modulator, is prohibited by World Anti Doping Agency (WADA) out-of-competition and in-competition. As it is extensively metabolized, further investigation of clomiphene metabolic profile will be essential to routine anti-doping analysis. The metabolic pathway and the different metabolites of clomiphene in human urine collected from three healthy volunteers during 1 week were studied by liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOFMS) based on accurate mass measurement. Seven unreported metabolites were identified and characterized, and all of the newly found urinary metabolites belonged to a new metabolic pathway (hydrogenation). An approach for the metabolism study of clomiphene and its analogs by LC-QTOFMS was presented. Two metabolites, 3,4-dihydroxy-dihydro-clomiphene (m/z 440.1991) and 3,4-dihydroxy-dihydro-deethyl-clomiphne (m/z 412.1674), are the potential biomarkers for monitoring oral administration of clomiphene in doping control. Verf.-Referat