Abstract

The administration of testosterone and its precursors is forbidden according to WADA Prohibited List (S1.1 .b. Endogenous AAS when administered exogenously). For determination of testosterone doping the ratio between testosterone (T) and epitestosterone (E) is used. The athlete sample will be deemed to contain testosterone as Prohibited Substance and an Atypical Finding will be reported if the ratio between testosterone and epitestosterone (T /E value) is higher than four. According to the WADA Technical Document - TD2004EAAS there are specific requirements for GC/MS measurement of the T/E value, concentration of testosterone and concentration of epitestosterone. As stated in the WADA document the T/E value is given by the peak area or peak height ratio of testosterone and epitestosterone (equivalent to the glucuronide) obtained by measuring the ion at m/z 432 by GC/MS analysis in a Single Ion Monitoring (SIM). The T/E value must be adjusted in the Screening Procedure or the Confirmation Procedure with help of appropriate standard. The deuterated internal standards are preferable and fulfill the criteria. As the reference compounds available have three deuterium atoms included in the molecules of both testosterone and epitestosterone, the ion at m/z 435 is used in GC/MS analysis in a SIM mode. We present here the statistical evaluation based on screening results of 4160 samples analyzed in our laboratory during 2008. Einleitung