Detection of His-tagged Long-R3-IGF-I in a black market product
Deutscher übersetzter Titel: | Nachweis von His-tagged Long-R3-IGF-I in einem Schwarzmarktprodukt |
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Autor: | Kohler, Maxie; Thomas, Andreas; Walpurgis, Katja; Terlouw, Koen; Schänzer, Wilhelm; Thevis, Mario |
Erschienen in: | Growth hormone & IGF research |
Veröffentlicht: | 20 (2010), 5, S. 386-390, Lit. |
Format: | Literatur (SPOLIT) |
Publikationstyp: | Zeitschriftenartikel |
Medienart: | Gedruckte Ressource |
Sprache: | Englisch |
ISSN: | 1096-6374, 1532-2238 |
DOI: | 10.1016/j.ghir.2010.07.001 |
Schlagworte: | |
Online Zugang: | |
Erfassungsnummer: | PU201011008443 |
Quelle: | BISp |
Abstract
Objective: Performance-enhancing substances are illicitly used in elite or amateur sports and may be obtained from the black market due to a cheaper and easier availability. Although various studies have shown that black market products frequently do not contain the declared substances, enormous amounts of illegally produced and/or imported drugs are confiscated from athletes or at customs with alarming results concerning the outcome of the analyses of the ingredients. This case report describes the identification of His-tagged Long-R3-IGF-I, which is usually produced for biochemical studies, in an injection vial.
Design: The ingredients were isolated by immunoaffinity purification and identified by nano-UPLC, high-resolution/high accuracy mass spectrometry of the intact and trypsinated substance and by an enzyme-linked immunosorbent assay.
Results: (Tandem) mass spectra characterized the protein as Long-R3-IGF-I with a His6-tag attached to the C-terminus by the linker amino acids Leu–Glu.
Conclusion: His-tags are commonly added to proteins during synthesis to allow a convenient and complete purification of the final product and His-tags are subsequently removed by specific enzymes when being attached to the N-terminus. The effects of His-tagged Long-R3-IGF-I in humans have not been elucidated or described and the product may rather be a by-product from biochemical studies than synthesized for injection purposes. Verf.-Referat