New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS
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| Deutscher übersetzter Titel: | Eine neue Probenvorbereitungsmethode für den Nachweis von Stanozolol und seinen Metaboliten mittels GC/MSMS und LC/ESI-MSMS |
|---|---|
| Autor: | Fußhöller, G.; Koch, A.; Mareck, U.; Thomas, A.; Schmechel, A.; Sigmund, G.; Thevis, Mario; Schänzer, Wilhelm |
| Erschienen in: | Recent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis ; 4th to 9th June 2006 |
| Veröffentlicht: | Köln: Sportverl. Strauß (Verlag), 2006, S. 229-238, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Sammelwerksbeitrag |
| Medienart: | Elektronische Ressource (online) Gedruckte Ressource |
| Sprache: | Englisch |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201001000239 |
| Quelle: | BISp |
Abstract
This paper describes a simple and fast sample preparation procedure allowing the simultaneous confirmation of stanozolol and its major urinary metabolites. The procedure consists of very efficient purification steps such as solid-phase extraction, several liquid-liquid extractions and acidic re-extraction followed by GC/MSMS and LC/ESI-MSMS detection, respectively. The sample preparation procedure allows the confirmation of stanozolol, 3’-OH-stanozolol, 4ß-OH-stanozolol and 16ß-OH-stanozolol in human urine. Based on SPE and LLE with subsequent re-extraction, this procedure makes it possible to generate highly purified extracts for GC/MSMS and LC/MSMS analysis, respectively. Excellent detection limits enable the long-term detection of stanozolol misuse in doping control. Particularly suitable for a prolonged traceability are the hydroxylated metabolites 4ß-OH-stanozolol and 16ß-OH-stanozolol. Aus dem Text