Changes of vinculin and extracellular matrix components following blunt trauma to rat skeletal muscle

Gespeichert in:
Bibliographische Detailangaben
Deutscher übersetzter Titel:Aenderungen von Vinculin und extrazellulaeren Matrixkomponenten nach stumpfem Muskeltrauma bei Ratten
Autor:Kami, Katsuya; Masuhara, Mitsuhiko; Kashiba, Hitoshi; Kawai, Yoshinori; Noguchi, Koichi; Senba, Emiko
Erschienen in:Medicine and science in sports and exercise
Veröffentlicht:25 (1993), 7, S. 832-840, Lit.
Format: Literatur (SPOLIT)
Publikationstyp: Zeitschriftenartikel
Medienart: Gedruckte Ressource
Sprache:Englisch
ISSN:0195-9131, 1530-0315
Schlagworte:
Online Zugang:
Erfassungsnummer:PU199310067223
Quelle:BISp

Abstract

The purpose of the present study was to determine changes in the localization of vinculin, Type IV collagen, laminin, and fibronectin during the damage-repair process in the skeletal muscle over several days following acute blunt trauma. A single blunt trauma to the gastrocnemius muscle of the rat was induced by applying 1.57 J of kinetic energy to one leg. By day 2 after trauma, vinculin had disappeared rapidly from the intracellular submenbraneous region corresponding to the costamere, whereas Type IV collagen and laminin persisted around the degenerating myofibers (corresponding to the basement membrane). Fibronectin could be observed filling the widened endomysium and in the ruptured myofibers. In the early phase of muscle regeneration (day 5 after trauma), newly synthesized vinculin, Type IV collagen, and laminin formed ring-like structures around the regenerating myotubes. Fibronectin could be observed filling the widened endomysium around the regenerating myotubes. Further, when small newly formed or splitted myofibers were observed in the areas of regenerating myofibers (day 10 after trauma), vinculin, Type IV collagen, laminin, and fibronectin were localized in specialized regions corresponding to the costamere, basement membrane, and endomysium. These results demonstrated that restoration of the costamere, basement membrane, and endomysium are essential to the maturation of regenerating myofibers after injury. Verf.-Referat