Intensity-dependent gene expression after aerobic exercise in endurance-trained skeletal muscle
Deutscher übersetzter Titel: | Intensitätsabhängige Genexpression nach aerobem Training in trainierten Skelettmuskeln |
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Autor: | Popov, Daniil V.; Makhnovskii, Pavel A.; Kurochkina, Nadia S.; Lysenko, Evgeny A.; Vepkhvadze, Tatiana F.; Vinogradova, Olga L. |
Erschienen in: | Biology of sport |
Veröffentlicht: | 35 (2018), 3, S. 277-289, Lit. |
Format: | Literatur (SPOLIT) |
Publikationstyp: | Zeitschriftenartikel |
Medienart: | Elektronische Ressource (online) Gedruckte Ressource |
Sprache: | Englisch |
ISSN: | 0860-021X, 2083-1862 |
DOI: | 10.5114/biolsport.2018.77828 |
Schlagworte: | |
Online Zugang: | |
Erfassungsnummer: | PU201810007124 |
Quelle: | BISp |
Abstract des Autors
We investigated acute exercise-induced gene expression in skeletal muscle adapted to aerobic training. Vastus lateralis muscle samples were taken in ten endurance-trained males prior to, and just after, 4 h, and 8 h after acute cycling sessions with different intensities, 70% and 50% · VO2max. High-throughput RNA sequencing was applied in samples from two subjects to evaluate differentially expressed genes after intensive exercise (70% · VO2max), and then the changes in expression for selected genes were validated by quantitative PCR (qPCR). To define exercise-induced genes, we compared gene expression after acute exercise with different intensities, 70% and 50% · VO2max, by qPCR. The transcriptome is dynamically changed during the first hours of recovery after intensive exercise (70% · VO2max). A computational approach revealed that the changes might be related to up- and down-regulation of the activity of transcription activators and repressors, respectively. The exercise increased expression of many genes encoding protein kinases, while genes encoding transcriptional regulators were both up- and down-regulated. Evaluation of the gene expression after exercise with different intensities revealed that some genes changed expression in an intensity-dependent manner, but others did not: the majority of genes encoding protein kinases, oxidative phosphorylation and activator protein (AP)-1-related genes significantly correlated with markers of exercise stress (power, blood lactate during exercise and postexercise blood cortisol), while transcriptional repressors and circadian-related genes did not. Some of the changes in gene expression after exercise seemingly may be modulated by circadian rhythm.