Identification of recombinant human EPO variants in greyhound plasma and urine by ELISA, LC-MS/MS and western blotting : a comparative study
Gespeichert in:
| Deutscher übersetzter Titel: | Identifizierung von rekombinanten humanen EPO-Varianten in Plasma und Urin von Windhunden durch ELISA, LC-MS/MS und Western Blot : eine vergleichende Untersuchung |
|---|---|
| Autor: | Timms, Mark; Steel, Rohan; Vine, John |
| Erschienen in: | Drug testing and analysis |
| Veröffentlicht: | 8 (2016), 1/2, S. 164-176, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Zeitschriftenartikel |
| Medienart: | Elektronische Ressource (online) Gedruckte Ressource |
| Sprache: | Englisch |
| ISSN: | 1942-7603, 1942-7611 |
| DOI: | 10.1002/dta.1835 |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201607005323 |
| Quelle: | BISp |
Abstract des Autors
The recombinant human erythropoietins epoetin alfa (Eprex®), darbepoetin (Aranesp®) and methoxy polyethylene glycol-epoetin beta (Mircera®) were administered to greyhounds for 7, 10 and 14 days respectively. Blood and urine samples were collected and analysed for erythropoietin by ELISA, LC-MS/MS and western blotting. Limits of confirmation in plasma for western blotting and LC-MS/MS methods ranged from a low of 2.5mIU/mL, and closely matched the sensitivity of ELISA screening.