Intense resistance exercise induces early and transient increases in ryanodine receptor 1 phosphorylation in human skeletal muscle
Deutscher übersetzter Titel: | Intensives Krafttraining erzeugt frühe und vorübergehende Erhöhungen bei der Ryanodin-Rezeptor 1-Phosphorylierung in der menschlichen Skelettmuskulatur |
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Autor: | Gehlert, Sebastian; Bungartz, Gerd; Willkomm, Lena; Korkmaz, Yüksel; Pfannkuche, Kurt; Schiffer, Thorsten; Bloch, Wilhelm; Suhr, Frank |
Beteiligte Person: | Dzeja, Petras |
Erschienen in: | PLoS one / Public Library of Science |
Veröffentlicht: | 7 (2012), 11, Art.-ID e49326; [12 S.], Lit. |
Format: | Literatur (SPOLIT) |
Publikationstyp: | Zeitschriftenartikel |
Medienart: | Elektronische Ressource (online) |
Sprache: | Englisch |
ISSN: | 1932-6203 |
DOI: | 10.1371/journal.pone.0049326 |
Schlagworte: | |
Online Zugang: | |
Erfassungsnummer: | PU201409009253 |
Quelle: | BISp |
Abstract des Autors
Background: While ryanodine receptor 1 (RyR1) critically contributes to skeletal muscle contraction abilities by mediating Ca2+ion oscillation between sarcoplasmatic and myofibrillar compartments, AMP-activated protein kinase (AMPK) senses contraction-induced energetic stress by phosphorylation at Thr172. Phosphorylation of RyR1 at serine2843 (pRyR1Ser2843) results in leaky RyR1 channels and impaired Ca2+homeostasis. Because acute resistance exercise exerts decreased contraction performance in skeletal muscle, preceded by high rates of Ca2+-oscillation and energetic stress, intense myofiber contractions may induce increased RyR1 and AMPK phosphorylation. However, no data are available regarding the time-course and magnitude of early RyR1 and AMPK phosphorylation in human myofibers in response to acute resistance exercise. Purpose: Determine the effects and early time-course of resistance exercise on pRyR1Ser2843 and pAMPKThr172 in type I and II myofibers. Methods: 7 male subjects (age 23±2 years, height: 185±7 cm, weight: 82±5 kg) performed 3 sets of 8 repetitions of maximum eccentric knee extensions. Muscle biopsies were taken at rest, 15, 30 and 60 min post exercise. pRyR1Ser2843 and pAMPKThr172 levels were determined by western blot and semi-quantitative immunohistochemistry techniques. Results: While total RyR1 and total AMPK levels remained unchanged, RyR1 was significantly more abundant in type II than type I myofibers. pRyR1Ser2843 increased 15 min and peaked 30 min (p<0.01) post exercise in both myofiber types. Type I fibers showed relatively higher increases in pRyR1Ser2843 levels than type II myofibers and remained elevated up to 60 min post resistance exercise (p<0.05). pAMPKThr172 also increased 15 to 30 min post exercise (p<0.01) in type I and II myofibers and in whole skeletal muscle. Conclusion: Resistance exercise induces acutely increased pRyR1Ser2843 and concomitantly pAMPKThr172 levels for up to 30 min in resistance exercised myofibers. This provides a time-course by which pRyR1Ser2843 can mechanistically impact Ca2+handling properties and consequently induce reduced myofiber contractility beyond immediate fatiguing mechanisms. Verf.-Referat