A sensitive flow injection chemiluminescence method for the determination of progesterone
Deutscher übersetzter Titel: | Eine sensible Fließinjektion-Chemilumineszenz-Methode für die Bestimmung von Progesteron |
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Autor: | Cao, Wei; Gong, Pixue; Liu, Wenwen; Zhuang, Mei; Yang, Jinghe |
Erschienen in: | Drug testing and analysis |
Veröffentlicht: | 5 (2013), 3-4, S. 242-246, Lit. |
Format: | Literatur (SPOLIT) |
Publikationstyp: | Zeitschriftenartikel |
Medienart: | Gedruckte Ressource Elektronische Ressource (online) |
Sprache: | Englisch |
ISSN: | 1942-7603, 1942-7611 |
DOI: | 10.1002/dta.299 |
Schlagworte: | |
Online Zugang: | |
Erfassungsnummer: | PU201306004236 |
Quelle: | BISp |
Abstract
A sensitive flow injection (FI) chemiluminescence (CL) method was developed for the determination of trace amounts of progesterone. This method was based on the luminescent properties of the tris(1,10-phenanthroline) ruthenium(II) - potassium permanganate (KMnO4) - progesterone in acidic medium sensitized by Na2SO3. With the peak height as a quantitative parameter applying optimum conditions, the relative CL intensity was linear with progesterone concentration in the range of 1.0 × 10−10 ∼ 6.0 × 10−9 g·ml−1 and 6.0 × 10−9 ∼ 4.0 × 10−8 g·ml−1 with a detection limit of 7.1 × 10−11 g·ml−1. The relative standard deviation (RSD) was 2.79% for 1.0 × 10−8 g·ml−1 progesterone (n = 11). The proposed method held low detection limit and was successfully applied to determination of progesterone in pharmaceutical preparations. The possible CL reaction mechanism was also discussed. Verf.-Referat