Application of high-throughput IEF-PAGE for EPO-doping testing
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| Deutscher übersetzter Titel: | Anwendung von Hochdurchlauf-IEF-PAGE in der EPO-Dopinganalytik |
|---|---|
| Autor: | Reichel, Christian |
| Erschienen in: | Drug testing and analysis |
| Veröffentlicht: | 4 (2012), 9-10 (30th Cologne Workshop: Advances in sports drug testing), S. 728-732, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Zeitschriftenartikel |
| Medienart: | Gedruckte Ressource Elektronische Ressource (online) |
| Sprache: | Englisch |
| ISSN: | 1942-7603, 1942-7611 |
| DOI: | 10.1002/dta.1411 |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201301000882 |
| Quelle: | BISp |
Abstract
Since its first publication in 2000, the isoelectric focusing (IEF) method of erythropoietin (EPO) used in doping control has been considered a procedure with relatively small sample number capacity. To overcome this limitation, a variation of the current protocol was evaluated, which uses double-sized gels with 48–120 wells plus three electrodes and hence multiplies the capacity of the electrophoretic chamber. With this modification up to 120 samples and standards can be run on a single gel – thus, making IEF-PAGE of EPO a high-throughput method. The protocol is ideally suited for large-scale screening purposes. Verf.-Referat