Di(2-ethylhexyl) phthalate metabolites as markers for blood transfusion in doping control : intra-individual variability of urinary concentrations
Deutscher übersetzter Titel: | Di(2-ethylhexyl)phthalat-Metaboliten als Marker für Bluttransfusionen in der Dopinganalytik : intraindividuelle Variabilität von Urinkonzentrationen |
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Autor: | Solymos, Emese; Guddat, Sven; Geyer, Hans; Thomas, Andreas; Thevis, Mario; Schänzer, Wilhelm |
Erschienen in: | Drug testing and analysis |
Veröffentlicht: | 3 (2011), 11/12 (29th Cologne Workshop: Advances in Sports Drug Testing), S. 892-895, Lit. |
Format: | Literatur (SPOLIT) |
Publikationstyp: | Zeitschriftenartikel |
Medienart: | Gedruckte Ressource Elektronische Ressource (online) |
Sprache: | Englisch |
ISSN: | 1942-7603, 1942-7611 |
DOI: | 10.1002/dta.377 |
Schlagworte: | |
Online Zugang: | |
Erfassungsnummer: | PU201203001755 |
Quelle: | BISp |
Abstract
To indicate homologous or autologous blood transfusion in sports drug testing, quantification of increased urinary concentrations of di(2-ethylhexyl) phthalate (DEHP) metabolites presents a promising approach; however, the possible intra-individual variation of the metabolite concentrations over time has not been well characterized. The aim of this study was to explore the intra-individual variability of urinary DEHP metabolites among seven volunteers without special occupational exposure to DEHP during one week (n = 253) in order to investigate the possibility of increased urinary concentrations of the metabolites caused by, for example, residential, dietary, or environmental exposure. Quantification of three DEHP metabolites – mono(2-ethylhexyl) phthalate, mono(2-ethyl-5-oxohexyl) phthalate, and mono(2-ethyl-5-hydroxyhexyl) phthalate – was accomplished after enzymatic hydrolysis of urinary glucuronide conjugates and direct injection using isotope-dilution liquid chromatography-tandem mass spectrometry. Although urinary concentrations of DEHP metabolites showed considerable intra-individual variation, no increased values were observed comparable to the concentrations measured in urine specimens collected after blood transfusion. Verf.-Referat