A novel chemiluminescence quenching method for determination of sulfonamides in pharmaceutical and biological fluid based on luminol–Ag(III) complex reaction in alkaline solution
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| Deutscher übersetzter Titel: | Eine neue Methode des Chemilumineszenz-Quenchings zum Nachweis von Sulfonamiden in pharmazeutischen und biologischen Flüssigkeiten basierend auf der Reaktion mit einem Luminol-Ag(III)-Komplex in alkalischer Lösung |
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| Autor: | Sun, Hanwen; Chen, Peiyun; Li, Liqing; Cheng, Peng |
| Erschienen in: | Drug testing and analysis |
| Veröffentlicht: | 3 (2011), 5, S. 300-305, Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Zeitschriftenartikel |
| Medienart: | Gedruckte Ressource Elektronische Ressource (online) |
| Sprache: | Englisch |
| ISSN: | 1942-7603, 1942-7611 |
| DOI: | 10.1002/dta.249 |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU201111009726 |
| Quelle: | BISp |
Abstract
A novel chemiluminescence (CL) quenching method for the determination of sulfonamides is proposed. The CL reaction between Ag(III) complex [Ag(HIO6)2]5− and luminol in alkaline solution was investigated. The quenching effect of sulfonamides on CL emission of [Ag(HIO6)2]5−–luminol system was found. Quenching degree of CL emission was proportional to sulfonamide concentration. The effects of the reaction conditions on CL emission and quenching were examined. Under optimal conditions, the detection limits (s/n = 3) were 7.2, 17 and 8.3 ng/mL for sulfadiazine, sulfameter, and sulfadimethoxine, respectively. The recoveries of the three drugs were in the range of 91.3–110% with RSDs of 1.9–2.7% for urine samples, and 106–112% with RSDs of 1.6–2.8% for serum samples. The proposed method was used for the determination of sulfadiazine at clinically relevant concentrations in real urine and serum samples with satisfactory results. Verf.-Referat