Screening for autologous blood transfusions
Deutscher übersetzter Titel: | Suche nach übereinstimmenden Bluttransfusionen |
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Autor: | Mørkeberg, J.S.; Belhage, B.; Ashenden, M.; Bornø, A.; Sharpe, K.; Dziegiel, M.H.; Damsgaard, R. |
Erschienen in: | International journal of sports medicine |
Veröffentlicht: | 30 (2009), 4, S. 285-292, Lit. |
Format: | Literatur (SPOLIT) |
Publikationstyp: | Zeitschriftenartikel |
Medienart: | Gedruckte Ressource Elektronische Ressource (online) |
Sprache: | Englisch |
ISSN: | 0172-4622, 1439-3964 |
DOI: | 10.1055/s-0028-1105938 |
Schlagworte: | |
Online Zugang: | |
Erfassungsnummer: | PU200905002784 |
Quelle: | BISp |
Abstract
The ratio between the amount of hemoglobin in the mature erythrocyte population and the reticulocytes (RBCHb:RetHb ratio) has previously been suggested as a marker to screen for EPO-abuse. We speculated that the reinfusion of blood would lead to a marked increase in this ratio, making it a valuable parameter in the screening for autologous blood doping. Three bags of blood (∼2010 +/- 11 g of Hb) were withdrawn from 16 males and stored at either −80°C (−80 T, n=8) or +4°C (+4 T, n=8) and reinfused 10 weeks or 4 weeks later, respectively. Seven subjects served as controls. Different erythrocyte parameters were measured on a hematological analyzer serially throughout and during a 4 week wash-out period. By using RBCHb:RetHb ratio cut-off limits of 145.7 (1:100) (‘suspicious') and 182.9 (1:1 000) (‘positive'), 35.4% (−80 T) and 19.6% (+4 T) of all samples obtained during a 4 week wash-out period were identified as ‘suspicious', and 18.8% (−80 T) and 4.3% (+4 T) as ‘positive'. In total, 7 out of 16 (43.8%) subjects had at least one sample exceeding 182.9. Compared to the currently used indirect parameters, the RBCHb:RetHb ratio is the best indicator of autologous blood doping after reinfusion, and the parameter could be used in a testing setting, once stability validation has been performed. Verf.-Referat