Belastungsinduzierte DNA-Effekte und Hämoxygenase-Expression in Leukozyten

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Bibliographische Detailangaben
Englischer übersetzter Titel:Exercise-induced DNA effects and expression of heme oxygenase in leucocytes
Autor:Nieß, Andreas Michael; Fehrenbach, E.; Veihelmann, S.; Passek, F.; Röcker, K.; Northoff, H.; Dickhuth, H.H.
Erschienen in:Deutsche Zeitschrift für Sportmedizin
Veröffentlicht:49 (1998), Sonderheft 1 (35. Deutscher Sportärztekongress Tübingen '97), S. 103-106, Lit.
Format: Literatur (SPOLIT)
Publikationstyp: Zeitschriftenartikel
Medienart: Gedruckte Ressource
Sprache:Deutsch
ISSN:0344-5925, 2627-2458
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Erfassungsnummer:PU199812305828
Quelle:BISp

Abstract des Autors

We studied the occurence of DNA damage in leucocytes and separated mononuclear cells (MN) of 10 subjects after a half marathon run (HM) using the single cell gelelectrophoresis (Comet-) assay. Additionally the cytoplasmic expression of the antioxidant stress-protein heme oxygenase-1 (HO-1) in lympho- (L), mono- (M) and granulocytes (G) was analysed by flow cytometry. Total leucocytes exhibited a greater frequency of DNA damaged cells (33%) 24 h after HM compared to separated mononuclear cells (15%), indicating a greater extent of DNA effects in G. Single MN showed a pronounced extent of DNA damage and the frequency of DNA effects in MN 24 h after HM was related to changes in plasma TNFa directly after exercise. Cytoplasmic HO-1 rose in L, G and M, but G and M exhibited a more pronounced expression 0, 3 and 24 h after HM. Differences in DNA damage and the expression of HO-1 between the leucocyte populations after HM let us suggest, that M and G are critical target cells susceptible to exercise induced oxidative stress. Verf.-Referat