Screening procedure for anabolic steroids - the control of the hydrolysis with deuterated androsterone glucuronide and studies with direct hydrolysis
| Deutscher übersetzter Titel: | Screening-Verfahren zum Nachweis anaboler Steroide - Kontrolle der Hydrolyse mit deuteriummarkiertem Androsteronglukuronid und Untersuchungen mit direkter Hydrolyse |
|---|---|
| Autor: | Geyer, Hans; Schänzer, Wilhelm; Marek-Engelke, U.; Nolteernsting, E.; Opfermann, G. |
| Herausgeber: | Schänzer, Wilhelm; Geyer, Hans; Gotzmann, Andrea; Marek-Engelke, U. |
| Erschienen in: | Recent advances in doping analysis (5) : Proceedings of the Manfred Donike Workshop ; 15th Cologne Workshop on Dope Analysis ; 23rd to 28th February 1997 |
| Veröffentlicht: | Köln: Sport u. Buch Strauß (Verlag), 1998, 1998. S. 99-101, Lit., Lit. |
| Format: | Literatur (SPOLIT) |
| Publikationstyp: | Sammelwerksbeitrag |
| Medienart: | Gedruckte Ressource |
| Sprache: | Englisch |
| ISBN: | 3890010164 |
| Schlagworte: | |
| Online Zugang: | |
| Erfassungsnummer: | PU199805301835 |
| Quelle: | BISp |
Abstract des Autors
For the deconjugation of steroid conjugates an enzymatic hydrolysis with beta-glucuronidase or beta-glucuronidase/arylsulfatase is performed. To control the hydrolysis step, we have synthesized [2,2,3,4,4-2H5]-androsterone glucuronide. This deuterated glucuronide is used as an internal standard and allows together with the unconjugated internal standard [2,2,4,4-2H4]-etiocholanolone a judgement of the completeness of the hydrolysis of steroid glucuronides. The control of the hydrolysis step makes it possible to perform a direct hydrolysis of steroid conjugates without a previous solid phase extraction (e.g. by Amberlite XAD-2 polystyrene resin). In several studies we could not find disadvantages of the direct hydrolysis regarding the detection of low amounts of anabolic steroids or quantitation of endogenous steroids. Verf.-Referat