Effect of physical exercise on in vitro production of interleukin 1, interleukin 6, tumor necrosis factor-alpha, interleukin 2 and interferon-gamma

Gespeichert in:
Bibliographische Detailangaben
Deutscher übersetzter Titel:Auswirkung von koerperlichem Training auf die in vitro Produktion von Interleukin 1, Interleukin 6, Tumor-Nekrose-Faktor-alpha, Interleukin 2 und Interferon-gamma
Autor:Haahr, P.M.; Pedersen, B.K.; Fomsgaard, A.; Tvede, N.; Diamant, M.; Klarlund, K.; Halkjar-Kristensen, J.; Bendtzen, K.
Erschienen in:International journal of sports medicine
Veröffentlicht:12 (1991), 2, S. 223-227, Lit.
Format: Literatur (SPOLIT)
Publikationstyp: Zeitschriftenartikel
Medienart: Gedruckte Ressource Elektronische Ressource (online)
Sprache:Englisch
ISSN:0172-4622, 1439-3964
DOI:10.1055/s-2007-1024672
Schlagworte:
Online Zugang:
Erfassungsnummer:PU199203049486
Quelle:BISp

Abstract

The present study was designed to examine the effect of physical exercise on production of interleukin-1 (IL-1), interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-alpha), interleukin-2 (IL-2) and interferon-gamma (IFN-gamma). Ten young, healthy volunteers underwent 60-min bicycle exercise at 75 of maximal oxygen uptake (VO2max). Blood samples were collected before and during the last minutes of exercise, as well as 2 h and 24 h later. Blood mononuclear cells (BMNC) were stimulated in vitro with either bacterial lipopolysaccharide or phytohaemagglutinin, and the supernatants were tested for the above-mentioned cytokines using bioassays as well as ELISA techniques. The production of IL-6 increased significantly 2 h after exercise, furthermore the production of IL-1-alpha and IL-1-gamma was enhanced, although only borderline significant. TNF-alpha, IL-2 and IFN-gamma did not fluctuate in relation to exercise. The increased amounts of IL-1 and IL-6 in the supernatants generated from a fixed number of BMNC are most likely explained by the increased percentage and absolute number of blood monocytes 2 h after exercise. IL-2 and IFN-gamma are mainly produced by CD4+ and CD16+ cells. During exercise the CD4+ subset decreases, while the CD16+ subset increases. The finding of unchanged production of IL-2 and IFN-gamma was therefore expected. Verf.-Referat