Effect of physical exercise on in vitro production of interleukin 1, interleukin 6, tumor necrosis factor-alpha, interleukin 2 and interferon-gamma
Deutscher übersetzter Titel: | Auswirkung von koerperlichem Training auf die in vitro Produktion von Interleukin 1, Interleukin 6, Tumor-Nekrose-Faktor-alpha, Interleukin 2 und Interferon-gamma |
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Autor: | Haahr, P.M.; Pedersen, B.K.; Fomsgaard, A.; Tvede, N.; Diamant, M.; Klarlund, K.; Halkjar-Kristensen, J.; Bendtzen, K. |
Erschienen in: | International journal of sports medicine |
Veröffentlicht: | 12 (1991), 2, S. 223-227, Lit. |
Format: | Literatur (SPOLIT) |
Publikationstyp: | Zeitschriftenartikel |
Medienart: | Gedruckte Ressource Elektronische Ressource (online) |
Sprache: | Englisch |
ISSN: | 0172-4622, 1439-3964 |
DOI: | 10.1055/s-2007-1024672 |
Schlagworte: | |
Online Zugang: | |
Erfassungsnummer: | PU199203049486 |
Quelle: | BISp |
Abstract
The present study was designed to examine the effect of physical exercise on production of interleukin-1 (IL-1), interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-alpha), interleukin-2 (IL-2) and interferon-gamma (IFN-gamma). Ten young, healthy volunteers underwent 60-min bicycle exercise at 75 of maximal oxygen uptake (VO2max). Blood samples were collected before and during the last minutes of exercise, as well as 2 h and 24 h later. Blood mononuclear cells (BMNC) were stimulated in vitro with either bacterial lipopolysaccharide or phytohaemagglutinin, and the supernatants were tested for the above-mentioned cytokines using bioassays as well as ELISA techniques. The production of IL-6 increased significantly 2 h after exercise, furthermore the production of IL-1-alpha and IL-1-gamma was enhanced, although only borderline significant. TNF-alpha, IL-2 and IFN-gamma did not fluctuate in relation to exercise. The increased amounts of IL-1 and IL-6 in the supernatants generated from a fixed number of BMNC are most likely explained by the increased percentage and absolute number of blood monocytes 2 h after exercise. IL-2 and IFN-gamma are mainly produced by CD4+ and CD16+ cells. During exercise the CD4+ subset decreases, while the CD16+ subset increases. The finding of unchanged production of IL-2 and IFN-gamma was therefore expected. Verf.-Referat