Human skeletal muscle Na,K-ATPase concentration quantified by 3H-ouabain binding to intact biopsies before and after moderate physical conditioning
Deutscher übersetzter Titel: | Konzentration der Na,K-ATPase im menschlichen Skelettmuskel quantifiziert mit Hilfe der 3H-Oubain-Bindung an intakte Biopsien vor und nach maessig intensivem koerperlichem Training |
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Autor: | Kjeldsen, K.; Norgaard, A.; Hau, C. |
Erschienen in: | International journal of sports medicine |
Veröffentlicht: | 11 (1990), 4, S. 304-307, Lit. |
Format: | Literatur (SPOLIT) |
Publikationstyp: | Zeitschriftenartikel |
Medienart: | Gedruckte Ressource Elektronische Ressource (online) |
Sprache: | Englisch |
ISSN: | 0172-4622, 1439-3964 |
DOI: | 10.1055/s-2007-1024812 |
Schlagworte: | |
Online Zugang: | |
Erfassungsnummer: | PU199201046364 |
Quelle: | BISp |
Abstract
The putative effect of moderate training on human skeletal muscle Na,K-ATPase concentration and thus on the capacity for active uptake of potassium was evaluated. In 15 conscripts the Na,K-pump concentration was determined in vastus lateralis muscle by measurement of 3H-ouabain binding to intact muscle biopsies before and after 10 weeks of physical training. All subjects had improved physical fitness, body weight was reduced by 3 (P < 0.001), Coopers test showed an improvement by 7 (P < 0.05) and leg circumference 10 cm above the knee joint had increased by 3 (P < 0.001). Mean Na, K-pump concentration +/- S.E.M. in vastus lateralis muscles was 308 +/- 13 (N = 15) and 300 +/- 7 (N = 15) pmol/g wet wt. (P < 0.60) before and after training, respectively. Thus, in human subjects moderate improvement of physical performance may occur without any change in skeletal muscle Na,K-pump concentration. It may be, however, that change in the acute regulation of skeletal muscle Na,K-ATPase - i.e. augmented activity of existing Na,K-pumps - may reduce exercise-induced rise in plasma potassium concentration after moderate physical conditioning. Since the circumference of the legs had increased, the total amount of Na,K-ATPase in the legs had probably increased. Hence, moderate training may induce muscle hypertrophy with a balanced synthesis of muscle mass and Na,K-pumps. Verf.-Referat