Purine nucleotides and AMP deamination during maximal and endurance swimming exercise in heart and skeletal muscle of rats

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Deutscher übersetzter Titel:Purin-Nukleotide und AMP-Desaminierung in Herz- und Skelettmuskulatur bei Ratten waehrend maximaler und ausdauernder Schwimmarbeit
Autor:Weicker, H.; Hageloch, W.; Luo, J.; Mueller, D.; Werle, E.; Sehling, K.M.
Erschienen in:International journal of sports medicine
Veröffentlicht:11 (1990), Suppl. 2, S. S68-S77, Lit.
Format: Literatur (SPOLIT)
Publikationstyp: Zeitschriftenartikel
Medienart: Gedruckte Ressource
Sprache:Englisch
ISSN:0172-4622, 1439-3964
Schlagworte:
AMP
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Erfassungsnummer:PU199105044620
Quelle:BISp

Abstract

The purine nucleotides, phosphocreatine (PCr), ammonia, and lactic acid were investigated in skeletal muscles of rats with prominent type I, type IIa, type IIb fibers, and the heart after exhaustive and endurance swimming tests. ATP, ADP, AMP, IMP, and PCr were determined by HPLC with UV detection in controls after maximal and endurance training for 6 weeks with or without a respective final test and also after final exhaustive or endurance test without preceding training. The swimming time in these tests was longer with than without training. A pronounced ATP decrease and a large increase in IMP, up to 4.9 micro-mol/g wet weight, were found in type IIb fibers after the maximal final test without preceding training. Compared with skeletal muscle, the IMP concentration in the heart was sinificantly lower after all exercise bouts, even though after maximal exercise AMP augmentation was highest, ATP reduction was greatest, and energy charge was lowest. The difference between the heart and skeletal muscle in the production of IMP indicates that despite AMP and ADP accumulation, myoadenylate deaminase (MAD) activity in the heart is considerably lower than in skeletal muscle, especially in type IIb fibers. Verf.-Referat (gekuerzt)